Adipocyte-specific adhesion molecule (ASAM), also known as ACAM and CLMP, is a type I transmembrane protein and a member of the CTX (cortical thymocyte marker in Xenopus) family within the immunoglobulin superfamily. ASAM protein is highly expressed in the small intestine and placenta, and is found at intermediate levels in the heart, skeletal muscle, colon, spleen, kidney, and lung, and appears in low levels in the liver and peripheral blood leukocytes as well. ASAM is a transmembrane component of tight junctions in epithelial cells that can mediate cell aggregation and regulate transepithelial resistance across polarized epithelial cells. In addition, its expression is strongly correlated with white adipose tissue (WAT) mass of human and rodents with obesity.
Product Name:
Rat ASAM Recombinant Protein (RPES0408)
Product Code:
RPES0408
Size:
100 µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
ASAM,Clmp ,Acam
Accession:
Q8K1G0
Sequence:
Met1-Met232
Fusion tag:
C-His
Activity:
Measured by the ability of the immobilized protein to support the adhesion of MS-1 cells. When 5 x 10E4 cells/well are added to Recombinant Rat ASAM coated plates (12.5 µg/mL with 100 ?L/well), 35-70% cells will adhere after 1 hour incubation at 37?.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method
Protein Construction:
A DNA sequence encoding the rat ASAM (Q8K1G0) (Met1-Met232) was expressed, fused with a polyhistidine tag at the C-terminus.
Purity:
> 85 % as determined by SDS-PAGE
Mol Mass:
25.6 kDa
AP Mol Mass:
37 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
