The RPA1 Polyclonal Antibody (CAB0990) is a highly specific tool for studying the role of the Replication Protein A1 (RPA1) in DNA replication and repair processes. This antibody, produced in rabbits, is specifically designed for use in Western blot applications and is highly reactive with human samples.RPA1 is a key component of the replication protein A complex, which is essential for DNA replication, recombination, and repair. By targeting RPA1, this antibody allows for precise detection and analysis of the protein in various cell types, making it an invaluable tool for researchers in the fields of molecular biology and cancer research.
Understanding the function of RPA1 is crucial for unraveling the mechanisms of DNA replication and repair, as well as exploring its potential as a therapeutic target in cancer treatment. With its high specificity and sensitivity, the RPA1 Polyclonal Antibody (CAB0990) is an essential tool for advancing research in these important areas of study.
Product Name:
RPA70/RPA1 Rabbit Polyclonal Antibody
SKU:
CAB0990
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 367-616 of human RPA70/RPA70/RPA1 (NP_002936.1).
This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways.
Purification Method:
Affinity purification
Gene ID:
6117
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using RPA70/RPA1 Rabbit pAb (CAB0990) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
