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Anti-SATB2 Antibody (CAB16721)

SKU:
CAB16721
Product Type:
Antibody
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-SATB2 Antibody CAB16721
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-SATB2 Antibody CAB16721
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Description

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Antibody Name:Anti-SATB2 Antibody
Antibody SKU:CAB16721
Antibody Size:50uL, 100uL
Application:WB IHC
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 540-620 of human SATB2 (NP_056080.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:1000 IHC 1:50 - 1:100
Reactivity:Human, Rat
Positive Samples:MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 540-620 of human SATB2 (NP_056080.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TIRR FLNL PQHE RDVI YEEE SRHH HSER MQHV VQLP PEPV QVLH RQQS QPAK ESSP PREE APPP PPPT EDSC AKKP RSRT K
Gene ID:23314
Uniprot:Q9UPW6
Cellular Location:
Calculated MW:69kDa/82kDa
Observed MW:82kDa
Synonyms:SATB2, GLSS
Background:This gene encodes a DNA binding protein that specifically binds nuclear matrix attachment regions. The encoded protein is involved in transcription regulation and chromatin remodeling. Defects in this gene are associated with isolated cleft palate and cognitive disability. Alternate splicing results in multiple transcript variants that encode the same protein.
UniProt Protein Function:SATB2: Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper- layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11). Defects in SATB2 are a cause of cleft palate isolated (CPI). A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis. A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22). Belongs to the CUT homeobox family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: nucleoplasm; transcription factor complex; nuclear matrix; cytoplasm; histone deacetylase complex

Molecular Function:protein binding; sequence-specific DNA binding; chromatin binding

Biological Process: chromatin remodeling; transcription, DNA-dependent; commitment of a neuronal cell to a specific type of neuron in the forebrain; cartilage development; neuron migration; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; palate development; embryonic pattern specification; osteoblast development; embryonic skeletal morphogenesis

Disease: Glass Syndrome

UniProt Code:Q9UPW6
NCBI GenInfo Identifier:13634020
NCBI Gene ID:
NCBI Accession:Q9UPW6.2
Molecular Weight:81kDa
NCBI Full Name:DNA-binding protein SATB2
UniProt Protein Name:DNA-binding protein SATB2
UniProt Synonym Protein Names:Special AT-rich sequence-binding protein 2
Protein Family:DNA-binding protein
UniProt Gene Name:SATB2  
UniProt Entry Name:SATB2_HUMAN
Anti-SATB2 Antibody (CAB16721)Western blot analysis of extracts of MCF7 cells, using SATB2 antibody (CAB16721) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5min.
Anti-SATB2 Antibody (CAB16721)Immunohistochemistry of paraffin-embedded human colon carcinoma using SATB2 Rabbit pAb (CAB16721) at dilution of 1:100 (40x lens).

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