The SCARA5 Antibody (PACO55766) is a valuable tool for researchers studying SCARA5, a cell surface receptor known for its role in immune regulation and inflammation. This polyclonal antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the SCARA5 protein, this antibody enables the detection and analysis of SCARA5 in a variety of cell types, making it an ideal choice for studies in immunology and cancer research.
SCARA5 is a key player in immune response modulation, with implications for diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions. By understanding the function of SCARA5, researchers can uncover potential therapeutic targets for manipulating immune responses in these disease contexts. The SCARA5 Antibody offers a reliable tool for investigating the role of SCARA5 in immune homeostasis and inflammation, leading to new insights and potential treatment strategies.
IHC image of PACO55766 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with PACO55766 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Ferritin receptor that mediates non-transferrin-dependent delivery of iron. Mediates cellular uptake of ferritin-bound iron by stimulating ferritin endocytosis from the cell surface with consequent iron delivery within the cell. Delivery of iron to cells by ferritin is required for the development of specific cell types, suggesting the existence of cell type-specific mechanisms of iron traffic in organogenesis, which alternatively utilize transferrin or non-transferrin iron delivery pathways. Ferritin mediates iron uptake in capsule cells of the developing kidney. Binds preferrentially ferritin light chain (FTL) compared to heavy chain (FTH1).
Synonyms:
Scavenger receptor class A member 5 (Scavenger receptor hlg), SCARA5
UniProt Protein Function:
SCARA5: Ferritin receptor that mediates non-transferrin- dependent delivery of iron. Mediates cellular uptake of ferritin- bound iron by stimulating ferritin endocytosis from the cell surface with consequent iron delivery within the cell. Delivery of iron to cells by ferritin is required for the development of specific cell types, suggesting the existence of cell type- specific mechanisms of iron traffic in organogenesis, which alternatively utilize transferrin or non-transferrin iron delivery pathways. Ferritin mediates iron uptake in capsule cells of the developing kidney. Binds preferrentially ferritin light chain (FTL) compared to heavy chain (FTH1). Belongs to the SCARA5 family. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, integralChromosomal Location of Human Ortholog: 8p21.1Cellular Component: cell surface; integral to plasma membraneMolecular Function: scavenger receptor activityBiological Process: receptor-mediated endocytosis; cellular iron ion homeostasis; regulation of gene expression; regulation of blood coagulation; endocytosis
