The Alpha-1 Antitrypsin (SERPINA1) Monoclonal Antibody (CAB4966) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in various applications such as Western blotting and immunohistochemistry.SERPINA1, also known as alpha-1-antitrypsin, is a crucial inhibitor of proteases that can cause tissue damage. Dysregulation of SERPINA1 has been implicated in a variety of diseases, including emphysema, liver cirrhosis, and even certain cancers.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
The protein encoded by this gene is a serine protease inhibitor belonging to the serpin superfamily whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. This protein is produced in the liver, the bone marrow, by lymphocytic and monocytic cells in lymphoid tissue, and by the Paneth cells of the gut. Defects in this gene are associated with chronic obstructive pulmonary disease, emphysema, and chronic liver disease. Several transcript variants encoding the same protein have been found for this gene.
Purification Method
Affinity purification
Gene ID
5265
RRID
AB_2863400
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Alpha-1 Antitrypsin (Alpha-1 Antitrypsin (SERPINA1)) Rabbit mAb (CAB4966) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
