Description
Sheep Cytokine Array (18 targets) (SARB0092)
The Sheep Cytokine Array (SARB0092) is a powerful research tool for studying the expression levels of 18 different cytokine targets in sheep samples. This array allows for simultaneous detection and quantification of multiple cytokines in a single experiment, providing valuable insights into immune responses and inflammatory processes in sheep.Each target on the array is carefully selected to cover a wide range of cytokine families, including interleukins, chemokines, and growth factors. By using this array, researchers can profile the cytokine expression patterns in various experimental conditions, such as infections, inflammatory diseases, and immune responses to vaccines.
The Sheep Cytokine Array is easy to use and provides reliable and reproducible results. It is suitable for a wide range of sample types, including serum, plasma, cell lysates, and tissue homogenates. With its high sensitivity and specificity, this array is an essential tool for studies in veterinary immunology, infectious diseases, and livestock health.Overall, the Sheep Cytokine Array (SARB0092) is a valuable resource for researchers interested in understanding the immune system of sheep and investigating the role of cytokines in health and disease in this important animal model.
| Product Sku: | SARB0092 |
| Size: | 2, 4, or 8 |
| Species Detected: | Sheep |
| Number of Targets Detected: | 18 |
| Gene Symbols: | CCL5, DCN, FRZB, IFNG, IL-4, IL13, IL17A, IL1A, IL1B, IL21|IL4, LIF, SPINK5, TNF, TNFA, TNFSF2, VEGF, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
AIF
| Cadherin-6 (KCAD)
| Decorin
| IFN alpha
| IFN-gamma
|
IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-4
| IL-13
| IL-17A
|
IL-21
| LEKTI (SPINK5)
| LIF
| MIG (CXCL9)
| RANTES (CCL5)
|
sFRP-3
| TNF alpha
| VEGF-A
| ||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Sheep Cytokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
