The SIRT1 Polyclonal Antibody is a vital tool for researchers studying SIRT1, a key protein involved in cellular regulation and aging processes. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting SIRT1 in human samples, making it an essential component in Western blotting experiments.SIRT1, a member of the sirtuin family of proteins, plays a crucial role in various biological processes, including metabolism, DNA repair, and stress responses. Its involvement in longevity and age-related diseases has sparked interest in exploring its potential as a therapeutic target.
By targeting SIRT1, researchers can gain valuable insights into its functions and molecular mechanisms, paving the way for the development of novel treatments for age-related conditions, metabolic disorders, and other diseases influenced by SIRT1 activity. This antibody is a valuable tool for scientists seeking to unravel the complex roles of SIRT1 in health and disease.
Product Name:
SIRT1 Polyclonal Antibody
SKU:
CAB21418
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human SIRT1 (NP_036370.2).
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
23411
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SIRT1 Rabbit pAb (CAB21418) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
_WB_01__37459.1701341374.jpg?c=2?imbypass=on "Western blot analysis of extracts of various cell lines, using SIRT1 antibody (CAB21418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
_WB_01__37459.1701341374.jpg?c=2 "Western blot analysis of extracts of various cell lines, using SIRT1 antibody (CAB21418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
