The Symmetric Dimethyl Histone H3 (R26) Polyclonal Antibody (CAB3153) is a valuable tool for researchers studying epigenetic modifications in histone proteins. Histone H3 methylation at arginine 26 is a key regulator of chromatin structure and gene expression. This antibody, raised in rabbits, specifically recognizes the symmetric dimethylation of histone H3 at arginine 26, allowing for accurate detection and analysis in various biological samples.Symmetric dimethyl histone H3 (R26) is known to play a role in transcriptional regulation, DNA repair, and other cellular processes. Dysregulation of this epigenetic mark has been linked to various diseases, including cancer and neurological disorders.
By targeting and detecting this specific modification, researchers can gain insights into the mechanisms underlying disease development and progression.Validated for use in Western blot applications, this antibody offers high specificity and sensitivity, making it an essential tool for studies in epigenetics, cancer research, and other areas of biomedical research. Gain a deeper understanding of histone modifications with the Symmetric Dimethyl Histone H3 (R26) Polyclonal Antibody (CAB3153).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Symmetric DiMethyl-Histone H3-R26 Rabbit pAb (CAB3153) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
