Anti-TriMethyl-Histone H3-K27 Antibody (CAB2363)
- SKU:
- CAB2363
- Product Type:
- Antibody
- Applications:
- WB
- IHC
- IF
- IP
- ChIP
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
Antibody Name: | Anti-TriMethyl-Histone H3-K27 Antibody |
Antibody SKU: | CAB2363 |
Antibody Size: | 20uL, 50uL, 100uL |
Application: | WB IHC IF IP ChIP ChIPseq |
Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
Host Species: | Rabbit |
Immunogen: | A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3 |
Application: | WB IHC IF IP ChIP ChIPseq |
Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 ChIPseq 1:20 - 1:100 |
Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
Positive Samples: | HeLa, NIH/3T3 |
Immunogen: | A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3 |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Gene ID: | 8290 |
Uniprot: | Q16695 |
Cellular Location: | Chromosome, Nucleus |
Calculated MW: | 15kDa |
Observed MW: | 17kDa |
Synonyms: | H3.4, H3/g, H3FT, H3t, HIST3H3, Histone H3, HIST1H3A |
Background: | Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interaction between G-actin and Cdk9 stimulated Pol II transcription elongation. ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and acetylized histone H3 binding to a transcriptional template through the interaction with histone acetyltransferase, p300. Taken together, our results suggested that actin participates in transcription elongation by recruiting Cdk9 for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling. |
UniProt Protein Function: | HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family. |
UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 1q42 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; DNA binding; histone binding; protein heterodimerization activity Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance |
NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q16695 |
NCBI GenInfo Identifier: | 18202512 |
NCBI Gene ID: | 8290 |
NCBI Accession: | Q16695.3 |
UniProt Secondary Accession: | Q16695,Q6FGU4, B2R5K3, |
UniProt Related Accession: | Q16695 |
Molecular Weight: | |
NCBI Full Name: | Histone H3.1t |
NCBI Synonym Full Names: | histone cluster 3, H3 |
NCBI Official Symbol: | HIST3H3  |
NCBI Official Synonym Symbols: | H3t; H3.4; H3/g; H3FT  |
NCBI Protein Information: | histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T |
UniProt Protein Name: | Histone H3.1t |
UniProt Synonym Protein Names: | H3/g |
UniProt Gene Name: | HIST3H3  |
UniProt Entry Name: | H31T_HUMAN |
Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. | |
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (A2363). | |
Immunohistochemistry of paraffin-embedded rat ovary using TriMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded human breast cancer using TriMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded mouse brain using TriMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol. | |
Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody at dilution of 1:100. Blue: DAPI for nuclear staining. | |
Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K27 antibody at dilution of 1:100. Blue: DAPI for nuclear staining. | |
Immunofluorescence analysis of U-2 OS cells using TriMethyl-Histone H3-K27 antibody at dilution of 1:100. Blue: DAPI for nuclear staining. | |
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K27 Rabbit pAb antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |