The Active Caspase-3 Monoclonal Antibody (CAB11021) is a high-quality antibody developed for reliable detection and analysis of target proteins. Caspase-3 is a key player in the execution phase of apoptosis, responsible for the cleavage of various cellular proteins. This monoclonal antibody, produced in mice, specifically targets active Caspase-3 in various cell types and tissues.With its high specificity and sensitivity, this antibody is ideal for Western blotting and immunohistochemistry applications, enabling accurate detection and analysis of active Caspase-3 levels.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Active Caspase-3 Monoclonal Antibody
SKU:
CAB11021
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0185
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CPP32, SCA-1, CPP32B, Active Caspase-3
Positive Sample:
Jurkat treated with Staurosporine
Cellular Localization:
Cytoplasm.
Calculated MW:
32kDa
Observed MW:
17kDa
The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Purification Method
Affinity purification
Gene ID
836
RRID
AB_2758369
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Jurkat cells, using Active Caspase-3 Rabbit mAb (CAB11021) at 1:500 dilution. Jurkat cells were treated with Staurosporine(1 μM) at room temperature for 3 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
