The 5-Methylcytosine (5mC) Monoclonal Antibody (CAB20599) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced in mouse, is optimized for detecting 5mC in various genomic DNA samples, making it a valuable asset for researchers in the fields of epigenetics and molecular biology.DNA methylation at the 5th position of cytosine residues (5mC) plays a crucial role in gene expression regulation, genomic stability, and cellular differentiation. Aberrant DNA methylation patterns have been linked to various diseases, including cancer, neurological disorders, and developmental abnormalities. The Anti-5-Methylcytosine (5mC) Antibody enables researchers to explore these epigenetic modifications and their impact on cellular processes, providing insights into disease mechanisms and potential therapeutic targets.
This antibody is validated for use in ELISA, DB applications and has demonstrated reactivity against Species independent samples.
Product Name:
5-Methylcytosine (5mC) Monoclonal Antibody
SKU:
CAB20599
Size:
20μL, 100μL
Reactivity:
Species independent
Clone Number:
ARC50801
Immunogen:
Chemical compounds corresponding to 5-Methylcytosine (5mC).
Tested Applications:
ELISADB
Recommended Dilution:
DB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
5mC, 5-Methylcytosine (5mC)
Observed MW:
Refertofigures
In the mammalian genome, DNA methylation is an epigenetic mechanism involving the transfer of a methyl group onto the C5 position of the cytosine to form 5-methylcytosine. DNA methylation regulates gene expression by recruiting proteins involved in gene repression or by inhibiting the binding of transcription factor(s) to DNA. During development, the pattern of DNA methylation in the genome changes as a result of a dynamic process involving both de novo DNA methylation and demethylation. As a consequence, differentiated cells develop a stable and unique DNA methylation pattern that regulates tissue-specific gene transcription.? Intriguingly, postmitotic neurons still express DNA methyltransferases and components involved in DNA demethylation. Moreover, neuronal activity can modulate their pattern of DNA methylation in response to physiological and environmental stimuli. The precise regulation of DNA methylation is essential for normal cognitive function.
Purification Method
Affinity purification
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Dot-blot analysis of 5-Methylcytosine (5mC) and unmodified cytosine using 5-Methylcytosine (5mC) Rabbit mAb antibody (CAB20599) at 1:1000 dilution. 5-Methylcytosine (5mC) : Biotin-5'CGATAACCACTAGT(5mC)3' unmodified cytosine : Biotin-5'CGATAACCACTAGTC3'
