Description
Anti-Human CD62L In Vivo Antibody - Low Endotoxin
Introducing the Anti-Human CD62L In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD62L protein, which is pivotal in facilitating leukocyte homing to peripheral lymph nodes, making it ideal for research in immunology and related fields. With a rat IgG2a isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), perfect for ELISA, flow cytometry, and other in vivo assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy.
Enhance your research with this reliable and versatile antibody. CD62L, also known as L-selectin, is a cell adhesion molecule expressed on the surface of leukocytes. It plays a significant role in the recruitment of lymphocytes, monocytes, and neutrophils to inflammatory sites and peripheral lymphoid tissues. By mediating the initial interactions between leukocytes and endothelial cells, CD62L is crucial in the immune response and inflammation processes.
| Product Name: | Anti-Human CD62L In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0121 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | DREG56 |
| Protein: | CD62L |
| Product Type: | Monoclonal Antibody |
| Synonyms: | TQ1, LAM1, LEU8, LNHR, LSEL, CD62L, LYAM1, PLNHR, LECAM1, L-selectin |
| Isotype: | Mouse IgG1 |
| Reactivity: | Human |
| Immunogen: | Concentrated supernatant from PMA-activated human peripheral blood leukocytes |
| Applications: | B, FC, IF, IHC FF, PhenoCycler®, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CODEX®, FC, IF, IHC FF, WB |
| Recommended Usage: | FC The suggested concentration for this DREG56 antibody for staining cells in flow cytometry is ≤ 0.5 µg per 106 in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this DREG56 antibody for use in western blotting is 1-10 µg/ml. |
| Reactivity: | Human |
| Host Species: | Mouse |
| Specificity: | Clone DREG56 recognizes an epitope on human CD62L. |
| Antigen Distribution: | CD62L is expressed on most circulating leukocytes, including neutrophils, monocytes, T, B and NK cells, and human embryo trophoblasts. |
| Immunogen: | Concentrated supernatant from PMA-activated human peripheral blood leukocytes |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD62L antibody, clone DREG56, recognizes CD62L, also known as L-selectin or LECAM-1, a 74-95 kDa type I transmembrane glycoprotein and cell adhesion molecule expressed on most circulating leukocytes1. Ligands for CD62L include CD34, GlyCAM-1, and MAdCAM-1, which require sialyation, fucosylation, and carbohydrate sulfation for recognition2,3. Ligand binding mediates tethering and rolling of leukocytes, facilitating entry into secondary lymphoid organs, including lymph nodes and Peyer’s patches, via high endothelial venules (HEVs)1. CD62L is also expressed on human embryo trophoblasts and has been proposed to play a role in human embryo implantation4.
| Technical Datasheet: | View |
| Protein: | CD62L |
| Function: | Leukocyte homing, leukocyte tethering, rolling |
| Ligand/Receptor: | CD34, GlyCAM, MAdCAM-1 |
| Research Area: | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity |
| Mouse IgG1 Isotype Control [HKSP] | |
|---|---|
| Clone | HKSP |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Low Endotoxin |
| Mouse IgG1 Isotype Control [HKSP84] | |
|---|---|
| Clone | HKSP84 |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Low Endotoxin |
