The IKZF3 Monoclonal Antibody (CAB8614) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. Several alternative transcripts encoding different isoforms have been described, as well as some non-protein coding variants.
This antibody is validated for use in WB, IHC-P, IP, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
IKZF3 Monoclonal Antibody
SKU:
CAB8614
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2781
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIPELISAIF-P
Recommended Dilution:
WB
1:500 - 1:1000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
IF-P
1:50 - 1:200
IHC-P
1:200 - 1:800
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AIO, IMD84, AIOLOS, ZNFN1A3, IKZF3
Positive Sample:
Raji, Mouse spleen, Mouse thymus, Rat lung, , Raji, Ramos
Cellular Localization:
Cytoplasm, Cytosol, Nucleoplasm, Nucleus.
Calculated MW:
58 kDa
Observed MW:
50-70 kDa
This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. Several alternative transcripts encoding different isoforms have been described, as well as some non-protein coding variants.
Purification Method
Affinity purification
Gene ID
22806
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using IKZF3 Rabbit mAb (CAB8614) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of various lysates using IKZF3 Rabbit mAb (CAB8614) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): U-937. Exposure time: 45 s.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using IKZF3 Rabbit mAb (CAB8614) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using IKZF3 Rabbit mAb (CAB8614) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunoprecipitation analysis of 300 μg extracts of Raji cells using 3 μg IKZF3 antibody (CAB8614). Western blot was performed from the immunoprecipitate using IKZF3 antibody (CAB8614) at a dilution of 1:500.
