Description
Anti-Mouse IL-4 [11B11] In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse IL-4 [11B11] In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the IL-4 protein, a cytokine essential for various immune responses, making it ideal for research in immunology and related fields. With a rat IgG1 kappa isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), perfect for ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy.
Enhance your research with this reliable and versatile antibody. IL-4 is a crucial cytokine involved in the regulation of immune responses, particularly in allergic reactions and the differentiation of naive helper T cells (Th0 cells) to Th2 cells. It plays a significant role in the immune system by promoting the proliferation of B cells and the production of IgE, as well as by modulating the activity of various other immune cells.
| Product Name: | Anti-Mouse IL-4 In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0086 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 11B11 |
| Protein: | IL-4 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Interleukin-4, BCGF, BCDF, B Cell Stimulating Factor, BSF-1, IaIF, HCGF, MCGF2, MFF, TCGF-2 |
| Isotype: | Rat IgG1 κ |
| Reactivity: | Mouse |
| Immunogen: | Partially Purified Native Mouse IL-4 |
| Applications: | CyTOF®, ELISA Cap, ELISPOT, FA, ICC, IHC, IP, N |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | CyTOF®, ELISA Cap, ELISPOT, FA, ICC, IHC, IP, N |
| Recommended Usage: | ELISA This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5-2.0 µg/ml. Titration of the reagent is recommended for optimal performance for each application. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone 11B11 recognizes an epitope on mouse IL-4. |
| Antigen Distribution: | IL-4 is secreted by Th2 cells, NKT cells, mast cells, eosinophils, and basophils. |
| Immunogen: | Partially Purified Native Mouse IL-4 |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
IL-4 antibody, 11B11, recognizes mouse IL-4, also known as B-cell differentiation factor (BCDF) and B-cell stimulatory factor (BSF1). IL-4 is a pleiotropic 20 kDa cytokine secreted by T helper 2 cells (Th2) cells, natural killer T (NKT) cells, mast cells, eosinophils, and basophils1,2. IL-4 signaling through the IL-4 receptor alpha chain (IL-4Ra) induces the differentiation of naive T cells to Th2 cells3,4 and stimulates B cell differentiation, proliferation, and class switching to IgG1 and IgE isotypes5. In addition, IL-4 is implicated in normal wound healing6,7 and airway inflammation in patients with asthma8.
| Technical Datasheet: | View |
| Protein: | IL-4 |
| Research Area: | Immunology |
