The Symmetric DiMethyl-Histone H3-R8 Polyclonal Antibody (CAB20820) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, specifically recognizes the symmetric dimethyl histone H3 R8 modification, a crucial mark associated with gene activation and transcriptional regulation. Validated for use in various applications such as Western blot and immunofluorescence, this antibody allows for the detection and analysis of the symmetric dimethyl histone H3 R8 modification in human samples. Its high reactivity and specificity make it an excellent choice for investigating the role of this epigenetic mark in processes such as chromatin remodeling, DNA repair, and cell differentiation.
This antibody is validated for use in WB, ChIP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method
Affinity purification
Gene ID
8290 8350
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Symmetric DiMethyl-Histone H3-R8 Rabbit pAb (CAB20820) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Symmetric DiMethyl-Histone H3-R8 antibody (CAB20820) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
