Description
system_update_altDatasheet
Canine Cytokine Array (40 targets) (SARB0008)
The Canine Cytokine Array (SARB0008) from AssayGenie is a powerful tool for researchers studying immune responses in dogs. This array allows for the simultaneous detection of 40 different cytokines, providing a comprehensive view of the immune system's activity in canine samples.This array is designed for use in various applications, including serum/plasma samples, cell culture supernatants, and tissue lysates. With high sensitivity and specificity, researchers can confidently analyze cytokine levels in samples from healthy and diseased dogs, shedding light on the underlying mechanisms of immune-mediated diseases.
By studying cytokine profiles in canine samples, researchers can gain valuable insights into the immune response in dogs, paving the way for the development of new diagnostic tools and therapies for veterinary medicine. Whether investigating inflammatory conditions, infectious diseases, or cancer in dogs, the Canine Cytokine Array from AssayGenie is a valuable resource for advancing canine health research.
| Product Sku: | SARB0008 |
| Size: | 2, 4, or 8 |
| Species Detected: | Canine |
| Number of Targets Detected: | 40 |
| Gene Symbols: | C1QTNF1, CCL2, CCL4, CSF2, CXCL1, CXCL8, DCN, EGFR, EPOR, FGF7|HGF, IFNG, IL10, IL12B, IL17A, IL1B, IL2, IL6, KITLG, LGALS3, MET, PDGFB, TNFRSF1A, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Cystatin C
| Decorin
| EGFR
| ErbB3
| Erythropoietin
|
FGF-7 (KGF)
| Galectin-3
| GASP-1 (WFIKKNRP)
| GM-CSF
| GRO alpha (CXCL1)
|
HGF
| HGFR
| IFN-gamma
| IGFBP-2
| IL-1 alpha (IL-1 F1) |
IL-1 beta (IL-1 F2)
| IL-1 Ra (IL-1 F3) | IL-10
| IL-12 p40
| IL-13 |
IL-17A
| IL-2
| IL-21 | IL-6
| IL-8 (CXCL8)
|
MCP-1 (CCL2)
| MIP-1 beta (CCL4)
| MMP-8
| NCAM-1 (CD56)
| Nope
|
PDGF-BB
| RAGE
| RANTES (CCL5) | Resistin
| SCF
|
TGF alpha
| TNF alpha
| TNF RI (TNFRSF1A)
| Trappin-2
| VEGF-A
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Canine Cytokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
