The Cathepsin L/V/K/H Monoclonal Antibody (CAB4986) is a high-quality antibody developed for reliable detection and analysis of target proteins. Cathepsins are lysosomal proteases that play key roles in various cellular processes, including protein degradation, antigen processing, and tissue remodeling.This antibody, derived from rabbits, is highly specific and reactive with human samples, making it an excellent tool for studying the expression and activity of Cathepsin L, V, K, and H in biological samples. Validated for use in immunohistochemistry and Western blot applications, this antibody enables researchers to detect and analyze Cathepsin proteins in various cell types and tissues.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cathepsin L/V/K/H Monoclonal Antibody
SKU:
CAB4986
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1276
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Additionally, this protein cleaves the S1 subunit of the SARS-CoV-2 spike protein, which is necessary for entry of the virus into the cell.
Purification Method
Affinity purification
Gene ID
1514 1515 1513 1512
RRID
AB_2863404
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cathepsin L/V/K/H Rabbit mAb (CAB4986) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Cathepsin L/V/K/H Rabbit mAb (CAB4986) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Cathepsin L/V/K/H Rabbit mAb (CAB4986) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Cathepsin L/V/K/H Rabbit mAb (CAB4986) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
