The Cullin 1 Monoclonal Antibody (CAB19034) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in various applications such as Western blotting.Cullin-1 is involved in the regulation of cell cycle progression, signal transduction, and tumorigenesis, making it a target of interest in cancer research. By detecting and analyzing the Cullin-1 protein in different cell types, researchers can gain insight into its function and potential therapeutic implications in cancer treatment.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cullin 1 Monoclonal Antibody
SKU:
CAB19034
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0436
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Cullin 1, CUL1, cullin-1, MGC149834, MGC149835
Positive Sample:
A-549, K-562, MCF7, NIH/3T3, Mouse liver, Mouse brain, Rat testis
Cellular Localization:
Cytosol, Nucleoplasm, Plasma Membrane.
Calculated MW:
90kDa
Observed MW:
90kDa
Predicted to enable ubiquitin protein ligase binding activity and ubiquitin-protein transferase activity. Involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process and protein ubiquitination. Located in plasma membrane. Part of Parkin-FBXW7-Cul1 ubiquitin ligase complex and SCF ubiquitin ligase complex.
Purification Method
Affinity purification
Gene ID
8454
RRID
AB_2862526
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cullin 1 Rabbit mAb (CAB19034) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
