D-Lactate Assay Kit (Colorimetric) (BA0097)

SKU:

BA0097

Product Type:

Assay

Detection Method:

Colorimetric

Instrument:

Microplate Reader

Sample Type:

Serum, Plasma, Cell Culture Media

Research Area:

TCA Cycle

Diabetes & Obesity

Food Safety & Analysis

Plant & Environmental Stress

Clinical Chemistry

Description

Description

D-Lactate Assay Kit - Information

Assay Genie's Colorimetric D-lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.

Applications

For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.

D-Lactate Assay Kit - Key Features

Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM D-lactate in 96-well plate assay.
Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed.
High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

D-Lactate Assay Kit - Data Sheet
Kit Includes	Assay Buffer: 10 mL NAD Solution: 1 mL Enzyme A: 120 mL MTT Solution: 1.5 mL Enzyme B: 120 mL Standard: 1.0 mL 20 mM D-lactate
Kit Requires	Pipeting (multi-channel) devices. Clear-bottom 96-well plates and plate reader.
Method of Detection	OD565 nm
Detection Limit	0.05 mM
Samples	Serum, plasma, cell culture media etc
Species	All
Protocol Length	20 min
Size	100 tests
Storage	Store all reagents at -20°C
Shelf Life	6 months

More Details

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.