Description
| Product Name: | D-Lactic Acid/Lactate Assay Kit - Colorimetric |
| Product Code: | MAES0015 |
| Product Size: | 96 Assays |
| Sample Type: | Serum, plasma, animal tissue |
| Assay Time: | 20 min |
| Instrument: | Microplate reader |
| Sensitivity: | 0.06 mmol/L |
| Detection Range: | 0.06-8.0 mmol/L |
| Recovery Rate: | 99 |
| Inter CV: | 7.7 |
| Inter CV: | 3.8 |
D-Lactic acid is an isomer of L-lactic acid. D-lactic acid is a product of bacterial metabolism. Due to the metabolism of pyruvaldehyde, D-lactic acid is usually present in the blood of mammals. The increase in D-lactic acid concentration may be due to the production by excessive gastrointestinal microbial. Subclinical elevation of D-lactic acid is an indicator of sepsis and trauma. The accumulation of D-lactic acid can lead to D-lactic acidosis, which is manifested as unlocalized nervous system symptoms and encephalopathy symptoms.
Using NAD+ as H+ receptor, D-lactate dehydrogenase (LDH) catalyzes the reaction of D- lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of D-lactic acid can be calculated.
| Shelf Life: | 6 Months |
| Storage: | 2-8°C |
| Shipping: | Gel Pack |
| Research Area: | Glycolysis & Carbohydrates |
