The [KO Validated] HuR/ELAVL1 Antibody (CAB6089) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to ELAVL1 and is validated for use in various applications, including Western blot and immunofluorescence.ELAVL1, also known as Hu antigen R (HuR), plays a crucial role in post-transcriptional regulation of gene expression by binding to target mRNAs and stabilizing them. Dysregulation of ELAVL1 has been linked to numerous diseases, including cancer, neurodegenerative disorders, and inflammatory conditions.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
[KO Validated] HuR/ELAVL1 Antibody
SKU:
CAB6089
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.
Purification Method
Affinity purification
Gene ID
1994
RRID
AB_2863522
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] HuR/ELAVL1 Rabbit pAb (CAB6089) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Western blot analysis of lysates from wild type (WT) and HuR/ELAVL1 knockout (KO) 293T cells, using [KO Validated] HuR/ELAVL1 Rabbit pAb (CAB6089) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human stomach using [KO Validated] HuR/ELAVL1 Rabbit pAb (CAB6089) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using [KO Validated] HuR/ELAVL1 Rabbit pAb (CAB6089) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
