The Equine Interleukin-18 (IL18) ELISA Kit is a powerful tool for accurately measuring interleukin-18 levels in equine serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it perfect for a variety of research applications.Interleukin-18 is a key cytokine involved in the immune response, inflammation, and various disease processes in horses. By measuring IL18 levels, researchers can gain valuable insights into the immune system and inflammatory processes in equine health and disease.
This kit is essential for studying conditions such as infections, autoimmune disorders, and inflammatory diseases in horses, and for developing potential therapies to improve equine health and well-being.Overall, the Equine Interleukin-18 (IL18) ELISA Kit is a valuable tool for researchers studying equine health and disease, offering accurate and reliable measurement of interleukin-18 levels in horse samples.
Product Name:
Equine Interleukin 18 (IL-18) ELISA Kit
Product Code:
HRDL00014
Size:
96 Assays
Target:
IL18
Synonyms:
IGIF, IL-1g, IL1F4, IL1-F4, Interferon-Gamma-Inducing Factor, Interleukin-1 Family Member 4, Iboctadekin, Interleukin-1 Gamma
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
