The FLG Monoclonal Antibody (CAB23193) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from mouse hybridoma cells, shows strong specificity for filaggrin in human samples and is validated for use in immunofluorescence and immunohistochemistry applications. By targeting and detecting filaggrin in skin tissue samples, this antibody enables detailed analysis of filaggrin expression and localization, making it invaluable for research in dermatology and skin biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
FLG Monoclonal Antibody
SKU:
CAB23193
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC60254
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.
Purification Method
Affinity purification
Gene ID
2312
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using FLG Rabbit mAb (CAB23193) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
