Glucose Dehydrogenase Assay Kit - Information
Assay Genie's non-radioactive, colorimetric GDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
Applications
For quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism.
Glucose Dehydrogenase Assay Kit - Key Features
- Fast and sensitive. Linear detection range (20 sample): 0.5 to 200 U/L for 15 min reaction.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Glucose Dehydrogenase Assay Kit - Data Sheet | |
| Kit Includes | Assay Buffer: 10 mL Diaphorase: 120 uL NAD/MTT: 1 mL Calibrator: 1.5 mL Substrate: 1 mL |
| Kit Requires | Pipetting devices and accessories (e.g. multi-channel pipettor), clear flat- bottom 96-well plates, centrifuge tubes and plate reader. |
| Method of Detection | OD565nm |
| Detection Limit | 0.5 U/L |
| Samples | Biological samples (e.g. plasma, serum, tissue and culture media). |
| Species | All |
| Protocol Length | 15 min |
| Size | 100 tests |
| Shelf Life | 6 months |
| Storage | Store all components at -20°C upon receiving |
More Details
GLUCOSE DEHYDROGENASE (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway.
