Description
| Product Name: | Glycogen Fluorometric Assay Kit |
| Product Code: | MAES0011 |
| Product Size: | 96 Assays |
| Sample Type: | Animal liver and muscle tissue |
| Assay Time: | 50min |
| Instrument: | Fluorescence microplate reader |
| Sensitivity: | 0.06 µg/mL |
| Detection Range: | 0.06-4.0 µg/mL |
| Inter CV: | 6.6 |
| Inter CV: | 3.4 |
Glycogen is mainly produced by liver and muscle. Glycogen content is mainly regulated by glycogen synthase and glycogen phosphorylase. In the liver, glycogen acts as a glucose store for other tissues and maintains blood glucose levels. In muscle, glycogen is mainly used as energy for the supply of adenosine triphosphate (ATP) during muscle contraction. Due to the lack of glucose-6-phosphatase, muscle glycogen cannot maintain the level blood glucose.
Glycogen produces glucose under the action of starch glycosidase, and glucose is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of the peroxidase, hydrogen peroxide be oxidized to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 587 nm is proportional to the glycogen content.
| Shelf Life: | 6 Months |
| Storage: | -20°C |
| Shipping: | Gel Pack |
| Research Area: | Glycolysis & Carbohydrates |
