The Histone H3 Antibody (CAB2348) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and reacts with histone H3 in a variety of samples, including human tissues. Validated for use in Western blot applications, this antibody binds specifically to histone H3, allowing for precise detection and analysis in a wide range of cell types.Histone H3 is a critical component of chromatin structure and plays a key role in regulating gene expression through modifications such as methylation and acetylation. Dysregulation of histone H3 has been implicated in various diseases, including cancer and neurological disorders, making it an important target for research.
This antibody is validated for use in WB, IHC-P, IP, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
Histone H3 Antibody
SKU:
CAB2348
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
IF-P
1:50 - 1:200
IHC-P
1:50 - 1:200
ChIP
3μg antibody for 5μg-10μg of Chromatin
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
H3t, H3.4, H3/g, H3FT, H3C16, HIST3H3, Histone H3
Positive Sample:
293F, NIH/3T3, PC-12
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
15kDa
Observed MW:
17kDa
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method
Affinity purification
Gene ID
8290 8350
RRID
AB_2631273
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Histone H3 Rabbit pAb (CAB2348) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Rat lung using Histone H3 Rabbit pAb (CAB2348) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human brain using Histone H3 Rabbit pAb (CAB2348) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Histone H3 Rabbit pAb (CAB2348) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse testis using Histone H3 Rabbit pAb (CAB2348) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 600 μg extracts of HeLa cells using 10ug Histone H3 antibody (CAB2348). Western blot was performed from the immunoprecipitate using Histone H3 antibody (CAB2348) at a dilution of 1:30000.
Immunoprecipitation analysis of 600 μg extracts of HeLa cells using 10ug Histone H3 antibody (CAB2348). Western blot was performed from the immunoprecipitate using Histone H3 antibody (CAB2348) at a dilution of 1:30000.
