The Hsp90beta Polyclonal Antibody (CAB21799) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the HSP90 protein, this antibody enables the detection and analysis of HSP90 in various cell types, making it an ideal choice for studies in cell biology, biochemistry, and cancer research.HSP90 is a crucial player in cellular homeostasis, as it assists in the folding of client proteins and helps to maintain proteostasis.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Hsp90beta Polyclonal Antibody
SKU:
CAB21799
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HSP84, HSPC2, HSPCB, D6S182, HSP90B, Hsp90β
Positive Sample:
K-562, DU 145, SW620, COS-7, NIH/3T3
Cellular Localization:
Cytoplasm, Melanosome.
Calculated MW:
83kDa
Observed MW:
Refertofigures
This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes.
Purification Method
Affinity purification
Gene ID
3326
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Hsp90β Rabbit pAb (CAB21799) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Hsp90β Rabbit pAb (CAB21799) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using Hsp90β Rabbit pAb (CAB21799) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human esophagus using Hsp90β Rabbit pAb (CAB21799) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of U2OS cells using Hsp90β Rabbit pAb (CAB21799). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
