The Hsc70/HSPA8 Monoclonal Antibody (CAB0415) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high specificity and sensitivity towards HSC70 in human samples, making it an ideal choice for various applications including Western blotting and immunohistochemistry.HSC70, a member of the HSP70 family, plays a key role in maintaining cellular homeostasis and protection against cellular stress. It is involved in numerous biological processes, including protein folding, transport, and degradation, as well as serving as a chaperone for newly synthesized proteins.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Hsc70/HSPA8 Monoclonal Antibody
SKU:
CAB0415
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0258
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
3312
RRID
AB_2861461
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Hsc70/HSPA8 Rabbit mAb (CAB0415) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
