Human Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) ELISA Kit
The Human Adenylate Cyclase Activating Polypeptide 1 (Pituitary ADCYAP1) ELISA Kit is a highly sensitive and specific assay designed for the quantitative detection of ADCYAP1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.Adenylate Cyclase Activating Polypeptide 1 (ADCYAP1) is a neuropeptide that plays a crucial role in various physiological processes, including neurotransmission, neuroprotection, and modulation of hormone release.
Dysregulation of ADCYAP1 has been associated with several neurological disorders, making it a valuable biomarker for studying these conditions and potentially developing new therapies.Overall, the Human Adenylate Cyclase Activating Polypeptide 1 (Pituitary ADCYAP1) ELISA Kit is a valuable tool for researchers looking to study the role of ADCYAP1 in health and disease.
Product Name:
Human Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
