The Human ADP (Acrp30) Adiponectin CLIA Kit is a cutting-edge assay designed for the precise measurement of ADP (Acrp30) Adiponectin levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.ADP (Acrp30) Adiponectin is a key adipokine involved in regulating insulin sensitivity and metabolic homeostasis. Dysregulation of ADP (Acrp30) Adiponectin levels has been linked to obesity, type 2 diabetes, cardiovascular diseases, and other metabolic disorders, highlighting its importance as a biomarker for studying these conditions and potential therapeutic interventions.
The Human ADP (Acrp30) Adiponectin CLIA Kit provides researchers with a valuable tool for investigating the role of ADP (Acrp30) Adiponectin in various physiological and pathological processes, ultimately advancing our understanding of metabolic diseases and paving the way for novel diagnostic and therapeutic strategies.
Product Name:
Human ADP/Acrp30 (Adiponectin) CLIA Kit
SKU:
HUES00004
Target:
Human ADP/Acrp30 (Adiponectin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ADP/Acrp30. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADP/Acrp30 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADP/Acrp30, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ADP/Acrp30. You can calculate the concentration of Human ADP/Acrp30 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
97-110
98-112
Average (%)
103
102
106
1:4
Range (%)
100-117
84-97
99-113
Average (%)
108
91
104
1:8
Range (%)
91-105
88-101
101-115
Average (%)
98
93
107
1:16
Range (%)
99-114
102-115
91-103
Average (%)
107
109
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
100
EDTA plasma (n=5)
100-112
106
Cell culture media (n=5)
96-111
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.35
4.95
24.81
2.5
5.3
25.21
Standard deviation
0.3
0.57
2.74
0.21
0.62
1.97
C V (%)
12.77
11.52
11.04
8.4
11.7
7.81
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ADP/Acrp30 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ADP/Acrp30 in samples. No significant cross-reactivity or interference between Human ADP/Acrp30 and analogues was observed.
