Description
Human Angiogenesis Array (20 targets) (SARB0019)
The Human Angiogenesis Array (SARB0019) offered by AssayGenie is a powerful tool for studying angiogenesis, the process of new blood vessel formation. This array includes 20 targets related to angiogenesis, allowing researchers to simultaneously detect and analyze multiple proteins involved in this crucial biological process.With a focus on angiogenesis-related molecules such as growth factors, receptors, and signaling proteins, this array provides a comprehensive view of angiogenic pathways and potential targets for therapeutic intervention. The array is compatible with human samples and can be used in a variety of research applications, including biomarker discovery, drug development, and disease studies.
By using the Human Angiogenesis Array, researchers can gain valuable insights into the complex mechanisms underlying angiogenesis and its role in various diseases, including cancer, cardiovascular disorders, and inflammatory conditions. With its high sensitivity and specificity, this array offers researchers a reliable and efficient way to study angiogenesis and advance our understanding of vascular biology.
| Product Sku: | SARB0019 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 20 |
| Gene Symbols: | ANG, CCL2, CCL5, CXCL1, CXCL2, CXCL3, CXCL5, CXCL8, EGF, FGF2|FIGF, IFNG, IGF1, IL6, LEP, PDGFB, PGF, TGFB1, THPO, TIMP1, TIMP2, TPO, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Angiogenin
| EGF
| ENA-78 (CXCL5)
| bFGF
| GRO alpha/beta/gamma
|
IFN-gamma
| IGF-1
| IL-6
| IL-8 (CXCL8)
| Leptin
|
MCP-1 (CCL2)
| PDGF-BB
| PLGF
| RANTES (CCL5)
| TGF beta 1
|
TIMP-1
| TIMP-2
| Thrombopoietin (TPO)
| VEGF-A
| VEGF-D
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Angiogenesis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
