Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) ELISA Kit
The Human ATPase Na+/K+ Transporting Alpha 1 Polypeptide (ATP1A1) ELISA Kit is a highly reliable and accurate tool for the detection of ATP1A1 levels in human samples such as serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.ATP1A1 is a key enzyme involved in the active transport of sodium and potassium ions across cell membranes, maintaining the electrochemical gradient necessary for various cellular functions.
Dysregulation of ATP1A1 activity has been linked to numerous diseases, including hypertension, heart failure, and neurological disorders, underscoring its importance as a potential diagnostic and therapeutic target.With its advanced technology and user-friendly protocol, the Human ATP1A1 ELISA Kit is an indispensable tool for researchers studying ion transport mechanisms, cellular signaling pathways, and disease pathogenesis. Stay ahead in your research with reliable results and precise quantification provided by this innovative ELISA kit.
Product Name:
Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
