The Human C1INH (Complement 1 Inhibitor) ELISA Kit is a specialized assay designed for the precise quantification of Complement 1 Inhibitor levels in human samples. C1INH plays a vital role in regulating the body's complement system, a key component of the immune response. By inhibiting the activation of complement proteins, C1INH helps maintain immune balance and prevent excessive inflammation. This ELISA kit enables accurate measurement of C1INH levels, offering insights into the immune response, inflammatory processes, and disorders related to complement system dysregulation. Accurate quantification of C1INH is essential for understanding its role in immune homeostasis, inflammatory diseases, and autoimmune conditions. The C1INH ELISA Kit from Assay Genie ensures exceptional sensitivity and specificity, delivering reliable and reproducible results. With robust performance and user-friendly protocols, this kit is suitable for a wide range of research and clinical applications. Trust Assay Genie's C1INH ELISA Kit for precise and dependable quantification of this crucial biomarker in your studies.
Product Name:
Human C1INH (Complement 1 Inhibitor) ELISA Kit
SKU:
AEES00088
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 ng/mL
Detection range:
31.25-2000 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.