The Human C5a Complement Component 5a CLIA Kit is specifically designed for the quantitative detection of C5a levels in human serum, plasma, and cell culture supernatants. This kit provides high sensitivity and accuracy, allowing for precise and reliable results in a variety of research applications.C5a is a key component of the complement system, a crucial part of the immune response that helps to eliminate pathogens and regulate inflammation. Abnormal levels of C5a have been linked to various diseases, including autoimmune disorders, inflammatory conditions, and infectious diseases.
By measuring C5a levels, researchers can gain valuable insights into the underlying mechanisms of these diseases and potentially identify new therapeutic targets.Overall, the Human C5a Complement Component 5a CLIA Kit offers a valuable tool for researchers studying the role of C5a in health and disease. Its high sensitivity, specificity, and reproducibility make it an essential asset for any laboratory conducting research in the field of immunology and inflammation.
Product Name:
Human C5a (Complement Component 5a) CLIA Kit
SKU:
HUES00164
Target:
Human C5a (Complement Component 5a)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human C5a. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C5a, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human C5a. You can calculate the concentration of Human C5a in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
100-118
98-112
Average (%)
105
108
104
1:4
Range (%)
100-112
101-113
84-95
Average (%)
106
107
90
1:8
Range (%)
93-107
103-117
95-108
Average (%)
99
109
101
1:16
Range (%)
88-104
98-115
87-100
Average (%)
95
105
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-114
104
EDTA plasma (n=5)
91-106
97
Cell culture media (n=5)
97-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.9
130.31
464.6
48.44
133.53
510.37
Standard deviation
5.7
9.17
44.97
4.92
14.93
38.48
C V (%)
10.78
7.04
9.68
10.16
11.18
7.54
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human C5a concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C5a in samples. No significant cross-reactivity or interference between Human C5a and analogues was observed.
