The Human C5a Complement Component 5a ELISA Kit is specifically designed for the precise measurement of C5a levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.C5a is a vital component of the complement system and plays a crucial role in inflammation and immune response. Dysregulation of C5a has been implicated in various inflammatory disorders, autoimmune diseases, and sepsis, making it a valuable biomarker for studying these conditions and exploring therapeutic interventions.
With its advanced technology and validated performance, the Human C5a Complement Component 5a ELISA Kit is an essential tool for scientists and researchers looking to investigate the role of C5a in health and disease. Obtain reliable data and insights with this innovative ELISA kit from Assay Genie.
Product Name:
Human C5a (Complement Component 5a) ELISA Kit
SKU:
HUES01457
Target:
Human C5a (Complement Component 5a)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C5a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C5a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C5a. You can calculate the concentration of Human C5a in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
93-107
96-111
Average (%)
94
99
103
1:4
Range (%)
93-105
81-94
85-99
Average (%)
99
86
90
1:8
Range (%)
90-104
81-93
86-101
Average (%)
97
86
92
1:16
Range (%)
87-100
80-93
81-94
Average (%)
94
86
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
96
EDTA plasma (n=5)
96-111
101
Cell culture media (n=5)
94-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
231.72
544.96
2063.64
250.03
497.26
1924.45
Standard deviation
12.95
24.52
70.37
15.75
27.95
84.48
C V (%)
5.59
4.5
3.41
6.3
5.62
4.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human C5a concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C5a in samples. No significant cross-reactivity or interference between Human C5a and analogues was observed.
