Description
Human Cancer Discovery Array (10 targets) (SARB0027)
The Human Cancer Discovery Array - 10 Targets (SARB0027) is a powerful tool for researchers looking to investigate key pathways involved in cancer development and progression. This array contains antibodies targeting 10 different proteins that play critical roles in cancer biology, including markers of cell proliferation, apoptosis, and metastasis.Each antibody in the array is rigorously validated for specificity and sensitivity, ensuring reliable and reproducible results. Researchers can use this array to analyze protein levels in a variety of cancer samples, providing valuable insights into the molecular mechanisms driving tumorigenesis.
With the Human Cancer Discovery Array - 10 Targets (SARB0027), researchers can accelerate their cancer research by efficiently analyzing multiple proteins simultaneously. This array is a valuable resource for studying cancer biology, identifying potential therapeutic targets, and developing personalized treatment strategies for cancer patients.
| Product Sku: | SARB0027 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 10 |
| Gene Symbols: | AFP, CA (19-9), CEACAM5, GDF15, HGF, KLK3, MUC16, SPP1, TIMP1, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Alpha-fetoprotein
| CA125
| CA19-9
| CEA
| GDF-15
|
HGF
| Osteopontin (SPP1)
| PSA-free
| TIMP-1
| VEGF-A
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cancer Discovery Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
