Description
system_update_altDatasheet
Human Chemokine Array (38 targets) (SARB0030)
The Human Chemokine Array - 38 Targets (SARB0030) is a powerful research tool for studying the interactions and functions of chemokines in human samples. This array allows for the simultaneous detection of 38 different chemokines in a single experiment, providing valuable insights into the inflammatory and immune responses in various disease states.With its high sensitivity and specificity, this array is designed to accurately quantify the levels of different chemokines, allowing researchers to compare the expression profiles of multiple targets in a single assay. This comprehensive approach enables a deeper understanding of chemokine signaling pathways and their impact on cell migration, activation, and immune cell recruitment.
The Human Chemokine Array - 38 Targets (SARB0030) is ideal for researchers studying cytokine networks, inflammation, and immune responses in diseases such as cancer, autoimmune disorders, and infectious diseases. By analyzing the levels of multiple chemokines simultaneously, this array provides a more complete picture of the complex interplay between chemokines and immune cells, offering valuable insights for the development of novel therapeutic strategies.
| Product Sku: | SARB0030 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 38 |
| Gene Symbols: | CCL1, CCL11, CCL13, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20|CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CCL3, CCL4, CCL5, CCL7, CCL8, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12, CXCL13, CXCL16, CXCL2, CXCL3, CXCL5, CXCL6, CXCL8, CXCL9, PPBP, XCL1 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
BLC (CXCL13)
| CCL28 (MEC)
| Ck beta 8-1 (CCL23)
| CTACK (CCL27)
| CXCL16
|
ENA-78 (CXCL5)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| Eotaxin-3 (CCL26)
| Fractalkine (CX3CL1)
|
GCP-2 (CXCL6)
| GRO alpha/beta/gamma
| GRO alpha (CXCL1)
| HCC-4 (CCL16)
| I-309 (TCA-3/CCL1)
|
I-TAC (CXCL11)
| IL-8 (CXCL8)
| IP-10 (CXCL10)
| Lymphotactin (XCL1)
| MCP-1 (CCL2)
|
MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
| MCP-4 (CCL13)
| MDC (CCL22)
| MIG (CXCL9)
|
MIP-1 alpha (CCL3)
| MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
| MIP-3 alpha (CCL20)
| MIP-3 beta (CCL19)
|
MPIF-1 (CCL23)
| NAP-2 (PPBP/CXCL7)
| PARC (CCL18)
| RANTES (CCL5)
| SDF-1 alpha (CXCL12 alpha)
|
SDF-1 beta (CXCL12 beta)
| TARC (CCL17)
| TECK (CCL25)
| ||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Chemokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
