The Human COL3 (Collagen Type III) ELISA Kit is designed for the quantitative determination of Collagen Type III levels in various human samples. Collagen Type III is a crucial structural protein that plays a significant role in the extracellular matrix, providing structural integrity and support to tissues. It is particularly abundant in tissues undergoing rapid growth or repair, contributing to the overall tissue architecture and function. Accurate measurement of Collagen Type III is essential as alterations in its levels can be indicative of pathological conditions, tissue remodeling, wound healing processes, and fibrosis-related disorders. This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. With stringent quality controls and ease of use, this kit delivers robust performance, making it well-suited for a wide range of research and clinical applications. Trust in Assay Genie's COL3 ELISA Kit for reliable and accurate quantification of this important biomarker in your studies.
Product Name:
Human COL3 (Collagen Type III) ELISA Kit
SKU:
AEES00085
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
