Description
system_update_altDatasheet
Human Cytokine Array (60 targets) (SARB0042)
The Human Cytokine Array - 60 Targets (SARB0042) is a powerful research tool that allows for the simultaneous detection of multiple cytokines in human samples. This array includes 60 cytokine targets, providing comprehensive coverage of key signaling molecules involved in immunology, inflammation, and cancer biology.With its high sensitivity and specificity, this array enables researchers to accurately measure cytokine levels in a variety of sample types, including serum, plasma, and cell culture supernatants. The array utilizes a sandwich ELISA format, ensuring reliable and quantitative results with minimal sample input.
By using the Human Cytokine Array - 60 Targets, researchers can gain valuable insights into the complex network of cytokine signaling pathways that drive immune responses and disease progression. This powerful tool is ideal for studying cytokine profiles in diverse research areas, such as autoimmune diseases, infectious diseases, and cancer immunotherapy.Overall, the Human Cytokine Array - 60 Targets (SARB0042) offers a cost-effective and efficient solution for high-throughput cytokine analysis, making it an essential resource for researchers conducting cutting-edge studies in immunology and related fields.
| Product Sku: | SARB0042 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 60 |
| Gene Symbols: | ADIPOQ, AGRP, ANGPT2, AREG, AXL, BTC, CCL16, CCL19, CCL25, CCL27|CCL28, CCL3, CCL4, CSF3, CXCL1, CXCL11, CXCL2, CXCL3, CXCL5, CXCL8, EGFR, FAS, FGF2, FGF4, FGF9, FIGF, HGF, ICAM1, ICAM3, IGF1R, IGFBP3, IGFBP6, IL11, IL12A, IL12B, IL17A, IL1R1, IL1RL1, IL2RA, IL6R, IL6ST, MIF, MST1, NGF, NTF4, OSM, PGF, PLAUR, THPO, TIMP1, TIMP2, TNFRSF10C, TNFRSF10D, TNFRSF11B, TNFRSF18, TNFRSF1A, TNFRSF1B, TNFSF18, TPO, TYRO3, VEGFA, XCL1 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Adiponectin (ACRP30)
| AgRP
| Angiopoietin-2
| Amphiregulin
| Axl
|
bFGF
| beta-NGF
| Betacellulin (BTC)
| CCL28 (MEC)
| CTACK (CCL27)
|
Dtk
| EGFR
| ENA-78 (CXCL5)
| Fas (TNFRSF6/Apo-1)
| FGF-4
|
FGF-9
| GCSF
| GITR Ligand (TNFSF18)
| GITR (TNFRSF18)
| GRO alpha/beta/gamma
|
GRO alpha (CXCL1)
| HCC-4 (CCL16)
| HGF
| ICAM-1 (CD54)
| ICAM-3 (CD50)
|
IGFBP-3
| IGFBP-6
| IGF-1 R
| IL-1 R4 (ST2)
| IL-1 R1
|
IL-11
| IL-12 p40
| IL-12 p70
| IL-17A
| IL-2 R alpha
|
IL-6 R
| IL-8 (CXCL8)
| I-TAC (CXCL11)
| Lymphotactin (XCL1)
| MIF
|
MIP-1 alpha (CCL3)
| MIP-1 beta (CCL4)
| MIP-3 beta (CCL19)
| MSP alpha/beta
| NT-4
|
Osteoprotegerin (TNFRSF11B)
| Oncostatin M
| PLGF
| gp130
| TNF RII (TNFRSF1B)
|
TNF RI (TNFRSF1A)
| TECK (CCL25)
| TIMP-1
| TIMP-2
| Thrombopoietin (TPO)
|
TRAIL R3 (TNFRSF10C)
| TRAIL R4 (TNFRSF10D)
| uPAR
| VEGF-A
| VEGF-D
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C7 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
