The Human DPP4 (Dipeptidyl Peptidase IV) ELISA Kit is expertly crafted for the quantitative assessment of DPP4 levels in diverse human biological samples. DPP4, a key enzyme involved in metabolism and immune regulation, plays a crucial role in several physiological processes, particularly in glucose metabolism and immune modulation. The accurate measurement of DPP4 levels is vital for understanding its implications in metabolic disorders, immune responses, and other pathophysiological conditions. Our ELISA kit offers outstanding sensitivity and specificity, guaranteeing precise and reproducible results essential for thorough investigations into the diverse functions of DPP4. Manufactured under strict quality control measures, this kit ensures robust performance and user-friendly operation, making it an ideal selection for research applications. Count on Assay Genie's DPP4 ELISA Kit for dependable and accurate quantification of this significant biomarker in your research studies.
Product Name:
Human DPP4 (Dipeptidyl Peptidase 4) ELISA Kit
SKU:
AEES00157
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
