Human EDN (EosinophILDerived Neurotoxin) ELISA Kit
The Human EDN (Eosinophil-Derived Neurotoxin) ELISA Kit is a powerful tool for the quantitative measurement of EDN levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reliable results, making it ideal for a wide range of research applications.EDN is a protein released by eosinophils, a type of white blood cell, and has been implicated in various inflammatory and allergic conditions. Its role in neurotoxicity and immune responses makes it a valuable biomarker for studying these processes and potentially developing new therapies.Overall, the Human EDN ELISA Kit offers researchers a precise and efficient method for studying the role of EDN in health and disease, providing valuable insights into inflammatory and immune-related conditions.
Product Name:
Human EDN (EosinophILDerived Neurotoxin) ELISA Kit
SKU:
HUES03216
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
