The Human Fe-Ferritin CLIA Kit is specifically designed for the quantitative measurement of ferritin levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, allowing for accurate and reproducible results, making it an ideal tool for a variety of research purposes.Ferritin is an important protein involved in iron storage and transport in the body. Abnormal ferritin levels can indicate conditions such as iron deficiency anemia, hemochromatosis, and other iron-related disorders.
Monitoring ferritin levels is essential in understanding and managing these conditions effectively.With the Human Fe-Ferritin CLIA Kit, researchers can confidently measure ferritin levels with precision and reliability, providing valuable insights into iron metabolism and related diseases. This kit is a valuable resource for studies focusing on iron homeostasis, inflammation, and various health conditions associated with ferritin dysregulation.
Product Name:
Human FE (Ferritin) CLIA Kit
SKU:
HUES00145
Target:
Human FE (Ferritin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FE. You can calculate the concentration of Human FE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
91-101
98-116
Average (%)
104
96
106
1:4
Range (%)
95-108
97-111
91-105
Average (%)
102
105
98
1:8
Range (%)
99-116
92-104
93-106
Average (%)
106
97
100
1:16
Range (%)
98-114
91-105
86-99
Average (%)
104
99
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
102
EDTA plasma (n=5)
99-112
106
Cell culture media (n=5)
99-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.68
179.57
890.55
84.34
175.84
910.59
Standard deviation
8.58
18.87
90.75
8.11
17.06
66.75
C V (%)
9.26
10.51
10.19
9.62
9.7
7.33
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FE in samples. No significant cross-reactivity or interference between Human FE and analogues was observed.
