Human FGF23 (Fibroblast Growth Factor 23) CLIA Kit
The Human FGF23 (Fibroblast Growth Factor 23) CLIA Kit is a powerful tool for the quantitative measurement of FGF23 levels in human samples, including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.FGF23 is a key regulator of phosphate metabolism and plays a critical role in bone mineralization and vitamin D metabolism.
Dysregulation of FGF23 has been linked to a variety of diseases, including chronic kidney disease, heart disease, and various metabolic disorders. By accurately measuring FGF23 levels, researchers can gain valuable insights into these conditions and potentially develop targeted therapies.Overall, the Human FGF23 CLIA Kit is an essential tool for researchers studying FGF23 and its role in various physiological and pathological processes.
Product Name:
Human FGF23 (Fibroblast Growth Factor 23) CLIA Kit
SKU:
HUES00655
Target:
Human FGF23 (Fibroblast Growth Factor 23)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FGF23. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGF23, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FGF23. You can calculate the concentration of Human FGF23 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
91-105
91-104
Average (%)
101
98
98
1:4
Range (%)
90-103
98-113
85-99
Average (%)
97
104
90
1:8
Range (%)
89-106
91-106
91-103
Average (%)
97
97
98
1:16
Range (%)
99-113
85-97
90-103
Average (%)
104
90
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
91
EDTA plasma (n=5)
91-105
98
Cell culture media (n=5)
86-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.61
79.41
223.95
25.49
71.56
238.82
Standard deviation
2.25
7.12
23.81
2.41
7.86
26.77
C V (%)
8.46
8.97
10.63
9.45
10.98
11.21
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FGF23 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGF23 in samples. No significant cross-reactivity or interference between Human FGF23 and analogues was observed.
