The Human Foxp3 (Forkhead Box Protein P3) CLIA Kit is specifically designed for the precise measurement of Foxp3 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Foxp3 is a key transcription factor involved in regulatory T cell function, crucial for maintaining immune tolerance and preventing autoimmune diseases.
Dysregulation of Foxp3 expression has been linked to various immune disorders and inflammatory conditions, making it a valuable biomarker for studying these diseases and potential therapeutic targets.By utilizing the Human Foxp3 CLIA Kit, researchers can gain valuable insights into the role of Foxp3 in immunological processes and disease pathogenesis, ultimately leading to the development of novel diagnostic tools and therapeutic interventions.
Product Name:
Human FOXP3 (Forkhead Box Protein P3) CLIA Kit
SKU:
HUES00649
Target:
Human FOXP3 (Forkhead Box Protein P3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FOXP3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FOXP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FOXP3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FOXP3. You can calculate the concentration of Human FOXP3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-102
87-100
97-111
Average (%)
97
92
105
1:4
Range (%)
103-116
92-105
93-108
Average (%)
109
100
100
1:8
Range (%)
99-113
92-109
88-100
Average (%)
106
99
94
1:16
Range (%)
101-115
100-115
92-110
Average (%)
106
108
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
100
EDTA plasma (n=5)
84-95
90
Cell culture media (n=5)
85-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
221.79
369.93
1826.76
225.79
374.26
1985.94
Standard deviation
22.05
41.43
160.02
19.98
33.68
218.85
C V (%)
9.94
11.2
8.76
8.85
9.0
11.02
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FOXP3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FOXP3 in samples. No significant cross-reactivity or interference between Human FOXP3 and analogues was observed.
