Human GLYAT Recombinant Protein (RPPB5815)
- SKU:
- RPPB5815
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q6IB77
- Research Area:
- Enzymes
Description
| Product Name: | Human GLYAT Recombinant Protein |
| Product Code: | RPPB5815 |
| Size: | 10µg |
| Species: | Human |
| Target: | GLYAT |
| Synonyms: | Glycine-N-acyltransferase, ACGNAT, GAT, CAT, AAc, HRP-1(CLP), Acyl-CoA: glycine amino acid N-acyltransferase, EC 2.3.1.13. |
| Source: | Escherichia Coli |
| Physical Appearance: | GLYAT is supplied as a sterile filtered clear solution. |
| Formulation: | GLYAT protein (0.5mg/ml) is supplied in 20mM Tris-HCl, pH-8, 1mM DTT and 20% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MMLPLQGAQM LQMLEKSLRK SLPASLKVYG TVFHINHGNP FNLKAVVDKW PDFNTVVVCP QEQDMTDDLD HYTNTYQIYS KDPQNCQEFL GSPELINWKQ HLQIQSSQPS LNEAIQNLAA IKSFKVKQTQ RILYMAAETA KELTPFLLKS KILSPSGGKP KAINQEMFKL SSMDVTHAHL VNKFWHFGGN ERSQRFIERC IQTFPTCCLL GPEGTPVCWD LMDQTGEMRM AGTLPEYRLH GLVTYVIYSH AQKLGKLGFP VYSHVDYSNE AMQKMSYTLQ HVPIPRSWNQ WNCVPL |
GLYAT is a mitochondrial acyltransferase that conjugates glycine with acyl-CoA substrates in the mitochondria. GLYAT is vital to the detoxification of endogenous and xenobiotic acyl-CoA's.
GLYAT Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 316 amino acids (1-296) and having a molecular mass of 36.0 kDa.The GLYAT is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GLYAT: Mitochondrial acyltransferase which transfers an acyl group to the N-terminus of glycine and glutamine, although much less efficiently. Can conjugate numerous substrates to form a variety of N-acylglycines, with a preference for benzoyl-CoA over phenylacetyl-CoA as acyl donors. Thereby detoxify xenobiotics, such as benzoic acid or salicylic acid, and endogenous organic acids, such as isovaleric acid. Belongs to the glycine N-acyltransferase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.3.1.71; Transferase; Mitochondrial; EC 2.3.1.13 Chromosomal Location of Human Ortholog: 11q12.1 Cellular Component: mitochondrial matrix; mitochondrion Molecular Function:glycine N-benzoyltransferase activity; protein binding; transferase activity, transferring acyl groups Biological Process: acyl-CoA metabolic process; glycine metabolic process; monocarboxylic acid metabolic process; xenobiotic metabolic process |
| NCBI Summary: | The glycine-N-acyltransferase protein conjugates glycine with acyl-CoA substrates in the mitochondria. The protein is thought to be important in the detoxification of endogenous and xenobiotic acyl-CoA's. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q6IB77 |
| NCBI GenInfo Identifier: | 311033446 |
| NCBI Gene ID: | 10249 |
| NCBI Accession: | Q6IB77.3 |
| UniProt Secondary Accession: | Q6IB77,O14833, Q96QK7, |
| UniProt Related Accession: | Q6IB77 |
| Molecular Weight: | 18,506 Da |
| NCBI Full Name: | Glycine N-acyltransferase |
| NCBI Synonym Full Names: | glycine-N-acyltransferase |
| NCBI Official Symbol: | GLYAT�� |
| NCBI Official Synonym Symbols: | CAT; GAT; ACGNAT�� |
| NCBI Protein Information: | glycine N-acyltransferase |
| UniProt Protein Name: | Glycine N-acyltransferase |
| UniProt Synonym Protein Names: | Acyl-CoA:glycine N-acyltransferase; AAc; Aralkyl acyl-CoA N-acyltransferase; Aralkyl acyl-CoA:amino acid N-acyltransferase; Benzoyl-coenzyme A:glycine N-acyltransferase; Glycine N-benzoyltransferase (EC:2.3.1.71 |
| Protein Family: | Glycine N-acyltransferase |
| UniProt Gene Name: | GLYAT�� |
| UniProt Entry Name: | GLYAT_HUMAN |
