Human GZMH Recombinant Protein (RPPB1788)
- SKU:
- RPPB1788
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P20718
- Research Area:
- Enzymes
Description
| Product Name: | Human GZMH Recombinant Protein |
| Product Code: | RPPB1788 |
| Size: | 10µg |
| Species: | Human |
| Target: | GZMH |
| Synonyms: | Granzyme H, Granzyme H (Cathepsin G-Like 2, ProteinH-CCPX), Cytotoxic T-Lymphocyte Proteinase, Cathepsin G-Like 2, CTSGL2, CCP-X,CSP-C, Cytotoxic T-Lymphocyte-Associated Serine Esterase 1, Cathepsin G-Like 2,Protein H-CCPX, Cytotoxin Serine Protease-C, Cytotoxic Serine Protease C,Protein H-CCPX, EC 3.4.21.79, EC 3.4.21, EC 3.4.21, CTLA1, CGL-2 , CGL2. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | GZMH protein solution (0.25mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | EEIIGGHEAK PHSRPYMAFV QFLQEKSRKR CGGILVRKDF VLTAAHCQGSSINVTLGAHN IKEQERTQQF IPVKRPIPHP AYNPKNFSND IMLLQLERKA KWTTAVRPLR LPSSKAQVKPGQLCSVAGWG YVSMSTLATT LQEVLLTVQK DCQCERLFHG NYSRATEICV GDPKKTQTGF KGDSGGPLVCKDVAQGILSY GNKKGTPPGV YIKVSHFLPW IKRTMKRLHH HHHH |
Granzyme H also known as GZMH belongs to the peptidase S1 family. GZMH is an essential part for HBV eradication. The HBx protein, which is required for the replication of HBV, is cleaved at Met(79) by GZMH. Furthermore, GZMH inhibitor can abolish GZNH- as well as lymphokine-activated killer cell-mediated HBx degradation and HBV clearance. A HBx-deficient HBV is resistant to GzmH- in addition to lymphokine-activated killer cell-mediated viral clearance.
GZMH produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 234 amino acids (19-246 a.a.) and having a molecular mass of 26.2kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). GZMH is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GZMF: Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Belongs to the peptidase S1 family. Granzyme subfamily. |
| UniProt Protein Details: | Protein type:Protease; EC 3.4.21.- Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: cytoplasm; intracellular membrane-bound organelle; membrane Molecular Function:serine-type endopeptidase activity Biological Process: immune response; protein processing |
| NCBI Summary: | This gene encodes a member of the peptidase S1 family of serine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a chymotrypsin-like protease. This protein is reported to be constitutively expressed in the NK (natural killer) cells of the immune system and may play a role in the cytotoxic arm of the innate immune response by inducing target cell death and by directly cleaving substrates in pathogen-infected cells. This gene is present in a gene cluster with another member of the granzyme subfamily on chromosome 14. [provided by RefSeq, Nov 2015] |
| UniProt Code: | P20718 |
| NCBI GenInfo Identifier: | 121590 |
| NCBI Gene ID: | 2999 |
| NCBI Accession: | P20718.1 |
| UniProt Secondary Accession: | P20718,Q6XGZ0, Q6XGZ1, G3V2C5, |
| UniProt Related Accession: | P20718 |
| Molecular Weight: | 12,609 Da |
| NCBI Full Name: | Granzyme H |
| NCBI Synonym Full Names: | granzyme H |
| NCBI Official Symbol: | GZMH�� |
| NCBI Official Synonym Symbols: | CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2�� |
| NCBI Protein Information: | granzyme H |
| UniProt Protein Name: | Granzyme H |
| UniProt Synonym Protein Names: | CCP-X; Cathepsin G-like 2; CTSGL2; Cytotoxic T-lymphocyte proteinase; Cytotoxic serine protease C; CSP-C |
| Protein Family: | Granzyme |
| UniProt Gene Name: | GZMH�� |
| UniProt Entry Name: | GRAH_HUMAN |
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