Human HBsAg (Hepatitis B surface antigen) ELISA Kit
The Human HBsAg (Hepatitis B Surface Antigen) ELISA Kit is a specialized assay designed for the quantitative detection of Hepatitis B surface antigen levels in various biological samples. HBsAg, a critical biomarker for Hepatitis B infection, serves as a key indicator in the diagnosis and monitoring of Hepatitis B virus activity. Accurate measurement of HBsAg levels is vital for diagnosing Hepatitis B infection, assessing disease progression, and evaluating treatment efficacy. This ELISA kit guarantees exceptional sensitivity and specificity, ensuring accurate and reproducible results for precise quantification of HBsAg. Manufactured under stringent quality control standards, the kit delivers robust performance, making it an ideal choice for both research studies and clinical diagnostics. Trust in the Human HBsAg ELISA Kit for dependable and accurate quantification of this essential viral marker in your investigative endeavors.
Product Name:
Human HBsAg (Hepatitis B surface antigen) ELISA Kit
SKU:
AEES00116
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
