The Human IgA (Immunoglobulin A) ELISA Kit is expertly crafted for the precise and quantitative assessment of IgA levels in a variety of human biological samples. Immunoglobulin A, a crucial antibody class, plays a vital role in the body's immune defense mechanisms, particularly in mucosal immunity, where it provides defense against pathogens trying to enter the body. Accurate measurement of IgA with our ELISA kit offers valuable insights into the immune response, antibody production, and mucosal immunity functions.
Upheld by exceptional sensitivity and specificity, our ELISA kit guarantees reliable and reproducible results. Manufactured under rigorous quality control standards, this kit delivers robust, high-performance outcomes and is user-friendly for both research and clinical applications. For precise and dependable quantification of this essential antibody, trust in our Human IgA ELISA Kit.
Product Name:
Human IgA (Immunoglobulin A) ELISA Kit
SKU:
AEES00107
Target:
Human IgA (Immunoglobulin A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IgA. You can calculate the concentration of Human IgA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
89-98
91-103
Average (%)
91
93
98
1:4
Range (%)
93-101
92-101
93-101
Average (%)
98
97
97
1:8
Range (%)
88-97
92-106
90-102
Average (%)
93
98
97
1:16
Range (%)
84-96
88-100
93-108
Average (%)
90
93
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-109
105
EDTA plasma (n=5)
86-98
94
Cell culture media (n=5)
94-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.85
12.26
49.18
4.15
12.3
45.61
Standard deviation
0.29
0.56
2.53
0.21
0.57
2.0
C V (%)
5.98
4.57
5.14
5.06
4.63
4.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IgA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IgA in samples. No significant cross-reactivity or interference between Human IgA and analogues was observed.
