The Human IL-1 alpha CLIA Kit is a powerful tool for the quantitative measurement of interleukin-1 alpha levels in human samples such as serum, plasma, and cell culture supernatants. This easy-to-use kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Interleukin-1 alpha is a key cytokine involved in inflammatory responses and immune regulation. Dysregulation of IL-1 alpha levels has been linked to various health conditions, including autoimmune diseases, inflammatory disorders, and cancer.
By accurately measuring IL-1 alpha levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.With its advanced technology and reliable performance, the Human IL-1 alpha CLIA Kit is an essential tool for researchers studying the role of interleukin-1 alpha in health and disease. Get precise, reliable results with this high-quality kit from Assay Genie.
Product Name:
Human IL-1 alpha CLIA Kit
SKU:
HUES00085
Target:
Human IL-1 alpha
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
2.34 pg/mL
Detection range:
3.91-250 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-1α. You can calculate the concentration of Human IL-1α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
99-110
98-113
Average (%)
98
104
104
1:4
Range (%)
94-107
98-115
93-106
Average (%)
100
105
99
1:8
Range (%)
100-113
88-102
96-107
Average (%)
108
94
102
1:16
Range (%)
92-103
98-109
87-102
Average (%)
98
104
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
93
EDTA plasma (n=5)
94-109
100
Cell culture media (n=5)
90-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
13.26
20.84
98.37
12.95
21.47
101.31
Standard deviation
1.65
2.33
7.22
1.2
2.06
9.84
C V (%)
12.44
11.18
7.34
9.27
9.59
9.71
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-1α concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-1α in samples. No significant cross-reactivity or interference between Human IL-1α and analogues was observed.
