Human MCP-4/CCL13 (Monocyte Chemotactic Protein 4) ELISA Kit
The Human MCP-4 (CCL13) Monocyte Chemotactic Protein-4 ELISA Kit is a powerful tool for accurate detection of MCP-4 levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit provides reliable and reproducible results, making it an essential resource for various research applications.MCP-4, also known as CCL13, is a key chemokine involved in the recruitment and activation of monocytes, playing a critical role in inflammatory responses and immune regulation. Dysregulation of MCP-4 levels has been linked to various diseases, including asthma, allergies, and autoimmune disorders, highlighting its importance as a potential biomarker for disease diagnosis and monitoring.By utilizing the Human MCP-4 (CCL13) Monocyte Chemotactic Protein-4 ELISA Kit, researchers can gain valuable insights into the role of MCP-4 in health and disease, paving the way for the development of novel therapeutic strategies and interventions.
Product Name:
Human MCP-4/CCL13 (Monocyte Chemotactic Protein 4) ELISA Kit
SKU:
HUES02239
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
